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This approach is explored with small molecule drug erastin (named after eradicator of RAS and ST-expressing cells) identified to efficiently kill human tumor cells without affecting their isogenic normal cell counterparts [160]

Proteins were transferred onto PVDF membranes, which were then blocked and incubated with primary antibodies against Neuroligin-3 (Synaptic System, #129321, 1:1,000) and Tubulin (Santa Cruz, #sc-5274, 1:2,000)