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Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere

Effects on cellular NAD+ and SAM pools in vitro

A BPA solution and working solution cannot be stored

Future priorities include optimizing biodegradability, conducting pilot trials, and integrating multi-omics data to advance precision therapy

Using a cell line of apoptosis induced by H 2 O 2 , it was observed that catalpol was able to suppress the down-regulation of Bcl-2, up-regulation of Bax, and the release of mitochondrial cytochrome c to the cytosol, in addition to attenuating the activation of caspase-3, PARP cleavage, and protect against apoptosis [204]
