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Lysate concentration was determined using Bradford assay (BSA standard) and diluted to 1 mg ml 1 and incubated with tetrazine-Cy5 (1 M, 30 minutes, 37 C) and then quenched with 5 l of 4 Laemmeli dye containing 6% -mercaptoethanol

[DOI] [PMC free article] [PubMed] [Google Scholar] 471.Wu Y, Torabi S-F, Lake RJ, Hong S, Yu Z, Wu P, et al

These intermediary toxins need to be further processed by the liver via Phase Two detoxification into water-soluble waste before they can be eliminated in the urine, bile, and stool

the solution would be placed in appropriate dispenser for ingestion as a liquid or administration as a spray

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