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Use new needles and the appropriate solvents under sterile procedures
We recently demonstrated that in HEK293 cells over-expressing IGF-1 isoforms the proIGF-1s are the main forms produced intracellularly, and for proIGF-1Ea both unglycosylated (~12 kDa) and glycosylated (~17 kDa) forms were detected 7
A standard calibration plot was generated at 750 nm using known concentrations of gallic acid
