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doi: 10.1126/science.3259727
Electrical signals were digitized and samples at 50 s intervals with Digidata 1440A and a Multiclamp 700B amplifier (Molecular Devices) using pCLAMP10.2 software
[DOI] [PubMed] [Google Scholar] Brombacher S., Owen S
Next, 5 L of 100 mM DTT (dithiothreitol) was added to reduce disulfide bonds, and the mixture was incubated at room temperature for 15 min
