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On the basis of CD and NMR studies: (i) the Cys2-Cys7 loop conformation has a short-span of helix (Ala5-Cys7)

Hosoya et al

Ice crystal formation during freezing can physically disrupt peptide structure, while thawing allows water to access reactive sites

We then evaluated the ability of the secreted FVII (and the other coagulation factors) to generate thrombin using a thrombin generation assay

Quality assessment The quality of each study was independently assessed by two reviewers using a modified Joanna Briggs Institute Checklist for Prevalence Studies 62
