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Optometry & Vision Science

The human GPX4 gene contains seven exons and six introns and can be expressed as three isoforms of the protein, mitochondrial (mGPX4, UniProt P36969-1), cytosolic (cGPX4, UniProt P36969-2) and nuclear (nGPX4) [23]

Funding This work was supported by the National Natural Science Foundation of China (82472525, 82272567 and 82502941), Suzhou Technology R&D Program (Healthcare Innovation) Project (SYW2025005), Suzhou Outstanding Youth Pilot Program (SSD2025027), Science and Education Project of Suzhou (QNXM2024001), BoXi Talent Cultivation Program (BXQN2024006), Suzhou Medical CollegeQilu Medical Research Program of Soochow University (24QL200203), the Project of MOE Key Laboratory of Geriatric Diseases and Immunology (KJS2502), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Postgraduate Research & Practice Innovation Program of Jiangsu Province (SJCX25_1798), Suzhou Municipal Medical and Health Science and Technology Innovation Project (SKY2021023), Ke Jiao Xing Wei Plan of Wujiang District (WWK202005)

The dried DNA adduct eluents were dansylated with 50 l of dansyl chloride (2 mg/ml) and 50 l of sodium bicarbonate buffer and incubated at 50 C for 5 min

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