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Margoshes and Vallee (1957) for the first time characterized MTs from horse kidneys as Cd-binding proteins

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Patients with Fitzpatrick IV-VI skin should be monitored for potential irritation during initial use, particularly in combination with other actives, and formulations should be pH-controlled (pH 3.5 or lower for efficacy without excessive irritation)

In this article: Nickel affinity chromatography Nickel bead binding capacity and buffer compatibility What the color of the nickel beads tells me about my purification Comparing with other affinity chromatography techniques Nickel is conjugated to agarose beads using one of two chelating agents: iminodiacetic acid (IDA) or nitrilotriacetic acid (NTA) (Figure 1)

[DOI] [PMC free article] [PubMed] [Google Scholar] 41.Shiraki N., Shiraki Y., Tsuyama T., Obata F., Miura M., Nagae G., Aburatani H., Kume K., Endo F., Kume S