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Mistake 1: Shaking the vial after adding water This is the single most common reconstitution error

An alternative approach that has been explored experimentally in the porcine ischemic injury model to limit formation of LA inhibitory fragments while maintaining the ability of LA to induce recovery of barrier function involves addition of small doses of LA over time and suggest that 0.1 M LA applied every 45 min is effective (49)

Sci Rep 12 , 484 (2022)

Assi R, Boddu P, Kadia T, Estrov Z, Garcia-Manero G, Ravandi F, Jabbour E, Takahashi K, Konopleva M, Andreeff M , Zahr AA, DiNardo C, Borthakur G, Jain N, Pemmaraju N, Yilmaz M, Hosrt N, Ning J, Pierce S, Wierda W, Cortex J, Kantarjian H, Daver N

Endothelin-1 (ET-1) increases NOX activity in human endothelial cells