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The metabolic profiles were augmented via a Monte Carlo-driven uncertainty propagation procedure (see Methods Estimation of Metabolic Profiles), in which each measurement was perturbed K pert times based on its estimated variance (Cramr-Rao lower bounds issued by the LCModel metabolite quantification maps, shown in Supplementary Fig

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Total RNA was used to synthesize cDNA using the SMART-Seq v4 full-length transcriptome analysis kit from Takara (product # 634888) using protocol B specified in the manual on page 12