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For expression into yeast mitochondria, p416TEFSu9DAAO(Pedre et al., 2023), which contains F 0 -ATPase subunit 9 (Su9) from Neurospora crassa -mitochondrial targetting sequence- and D-amino acid oxidase (DAAO) from Rhodotorula gracilis , was NcoI/HindIII digested to release DAAO and clone the codon-optimized Cystector C-terminally of Su9 via HiFi For mammalian cell expression, the final Cystector from the E

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