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This occurs in about 15% of children with NF1

Next, the cells were resuspended at a concentration of 1 10 6 cells/mL in Maxpar Water containing a 1:10 dilution of EQ Four Element Calibration Beads (Standard BioTools) and filtered through a 35 m nylon mesh filter cap (Corning, Falcon)

This reaction can be combined with strain-promoted azide-alkyne click (SPAAC) and inverse-electron-demand DielsAlder (iEDDA) reactions to achieve dual functionalization of proteins in a sequential simple one-pot reaction

Cellular signaling