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Muscle crush injury Right hind limbs were shaved, and using special system a force of 0.727 Ns/cm2 was delivered to a maximum diameter of gastrocnemius muscle complex 2 cm proximal to the insertion of the Achilles tendon (12)
Samples were then dried under a nitrogen gas stream and redissolved in 100 l (for serum) or 500 l (for liver) of 1:1 isopropanol:methanol for LCMS analysis 15

doi: 10.1016/j.intimp.2020.106765 53 FengZ.ZhangB

Methylmalonic acid is especially helpful when standard B12 levels appear falsely elevated but symptoms remain

1b (see also Supplementary Tables 3-4)
