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[5] After completion of this reaction, glutathione reductase recycles oxidized glutathione back to the reduced form so that it again can be picked up by GSTs

We reported the first reversible reaction-based small-molecule fluorescent GSH probe (ThiolQuant Green) that can perform single-point quantification of GSH levels in living cells 15

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We analyzed three different endogenous controls, Gadph, Actb, and Rn18s , and several genes of interest, Vegfr2, Src, Nos2, Nos1, Nos3, Akt1, Kras, Mapk1, Srf, Foxo1, Nfkb1, and Egr1

Paired t-test analysis was used for before-and-after comparisons of continuous data