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If deterioration appears, return to the frequency that maintained results

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Afterward, all the systems were subjected to a NVT (constant number of particles, volume and temperature) equilibration phase for 125,000 steps, using the V-rescale temperature-coupling method, with constant coupling of 1 ps at 303.15 K (Bussi et al

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mRNA was purified by NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490S) from DNAse treated total RNA