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Load a half-area, black, round-bottom, polystyrene, 96-well plate with duplicate 50 l samples of the following in Ca 2+ -free CLM: Serially diluted competing ligand (e.g., IP 3 , final concentrations 3 nM-150 M), FITC-IP 3 (final concentration 0.5 nM) and NT (from Step 40, final concentration 80 nM)

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Circadian gene expression and BMAL1 acetylation are disturbed in liver-specific SIRT1 mutant mice (Yang et al., 2017)

In myeloma, HCQ combined with bortezomib improved the efficiency of proteasome inhibitors by causing the accumulation of misfolded proteins, with 45% of patients showing stable disease
