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doi: 10.1186/cc13966 232 KovtunAMessererDAScharffetter-KochanekKHuber-LangMIgnatiusA
Subnormal alkaline phosphatase levels may indicate the presence of hypophosphatasia, Wilsons disease, deficiencies of divalent ions, or malnutrition
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Western blot analysis Cells were lysed in SDT buffer (4 % (w/v) SDS, 100 mM Tris/HCl, 1 mM DTT, pH 7.6) and denatured at room temperature for 30 min
Physical examination was consistent with phrynoderma
