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10.1093/ndt/gfw084 2 AnX.ZhangL.YuanY.WangB.YaoQ.LiL.et al (2017)

coli lacZ ORFs via fusion PCR [13], and the resulting aatJ - lacZ and aatQ - lacZ fusions were gel purified and subsequently cloned into pCR-Blunt (Invitrogen) to yield pJMS07 and pJMS08, respectively

Metformin, a first-line antidiabetic agent, has been investigated as a strategy to prevent glycocalyx degradation in a diabetic model (78)

1D), XM_029983790 (Fig

Site-directed mutagenesis and in vitro enzyme assays and quantification of FNSII proteins Each site-directed mutagenesis version of the target proteins was prepared as described according to the manual for the Fast Mutagenesis System (Transgen, China) and subcloned into the pYeDP60 vector