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Think of this as an internal thermostat for cellular health

O., Scherr, J

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For qRT-PCR assay, 1 g of purified RNA was reverse-transcribed into cDNA with PrimeScript RT reagent Kit (#RR047A, Takara) and quantitative real-time PCR was performed using Star Lighter SYBR Green qPCR Mix (#FS-Q1001, Beijing Foreverstar Biotech Co., Ltd) and run on Bio-Rad CFX96 Touch real-time PCR detection system according to the manufacturer's instructions

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