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Importance of purity evaluation and the potential of quantitative 1H NMR as a purity assay: Miniperspective

Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

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[DOI: 10.1136/bmj.c365] [DOI] [PubMed] [Google Scholar] Lam 2013 Lam JR, Schneider JL, Zhao W, Corley DA

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