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In humans, the CYP450 enzyme that predominately catalyses the formation of NAPQI is CYP2E1, although in vitro CYP3A4, CYP2D6 and CYP1A2 have also been implicated in the formation of the toxic metabolite [18,19,20]

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PMID 9786866

Further supporting the role of LA as a zonulin inhibitor, one study found that LA treatment reduced both rearrangement and redistribution of actin and the tight junction-associated protein zonula occludens-1 (ZO-1) in Caco-2 brush-border-expressing cells that were exposed to AT-1002, a synthetic hexamer peptide derived from the active Zot fragment that has been shown to increase tight junction permeability (24)

Combing B12 shots with a healthy diet and workout regime can give you great weight loss results