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DOI: Published: Publisher Name: Springer, Cham Print ISBN: 978-3-031-50418-1 Online ISBN: 978-3-031-50419-8 eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0) Keywords Antedrugs Bioactivation Biotransformation Codrugs Conjugation CYP3A4 CYP450 Cytochrome P450 Drug biotransformation Drug disposition Drug elimination Drug metabolism Efflux transporters Enzyme induction Enzyme inhibition Functionalization reactions Glucuronide Liver Metabolite Mutual prodrugs Personalized Medicine Phase I reactions Phase 1 reactions Phase II reactions Phase 2 reactions Precision Medicine Prodrugs Soft drugs Stratified Medicine Synthetic reactions

K.KumarA.WangL.RenY.et al (2021)

The mutations generated by Cas9 at the target exons were obtained by preparing genomic DNA from the selected clones and amplifying the exons with the primers: Fwd (hA5-ck1): 5-TCTTGTATCTGTACCTCTGA-3