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confirmed that ferroptosis was involved in kidney injury in STZ-induced type 1 diabetic mice and db/db mice[68]

The cells were incubated overnight at 37 C on Luria-Bertani-ampicillin agar plates and transformants were selected and grown overnight in 50 mL of LB medium containing 100 g/mL of ampicillin at 37 C

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Furthermore, excess Fe 2+ can act as a cofactor for lipid peroxidation enzymes, increasing lipid peroxidation, disrupting cell membrane phospholipids (mPLs), and triggering ferroptosis (Zou et al., 2020a