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Initial assessment of rats subjected to two ligations at the left colic artery and vein for 15 min, followed by reperfusion for next 15 min (IC + RL rats) Regular course (saline bath, control): After a saline bath was applied to the reperfused colon segment, the arcade vessels were poorly established from proximal to distal arcades

The result of the loss of water (and overall volume) leads to the activation of the thirst mechanism ( polydipsia )

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therefore, they have an essential requirement for nutrients present only in animal tissues [8]