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1 (C3008MP, ThermoFisher) in the presence of 3 M ionomycin and 3 M thapsigargin

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The tagged proteins are then passed through a column of beads containing covalently attached, immobilized nickel(II) (Ni 2 + .) This His-tag binds tightly to the immobilized metal ions because the side chain of Histidine, imidazole, has a specific binding affinity to metal ions (in this case, nickel II)

Inhibition of ACSL4 expression or knockdown of LPCAT3 may be key mechanisms for ultimately regulating ferroptosis through different signaling pathways