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GST tagged fusion proteins bind to glutathione agarose and, after washing, are eluted with elution buffer with excess glutathione Protein refolding Reducing agents are common additions to protein purification buffers because they help keep many proteins from forming unnatural disulfide bonds and aggregating
However, the function of MSC can vary according to cell culture conditions, donor age, and heterogeneity of the MSC population, resulting in unregulated MSC quality control

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