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Modifications after nisin synthesis are dehydration (Fig

The "standard" protocol involves ingestion of either placebo (PL) or L-carnitine (LC) in the form of 2 g 60 min before testing

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Immunoblotting For protein extraction, the cells were washed twice with ice-cold 1 PBS and lysed in 50 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, supplemented with protease and phosphatase inhibitors followed by sonication