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The following day, L1 larvae were transferred to NGM plates at ZT1 and were grown for 48 h to the L4 stage under the same LD/CW cycle

If the assay were measuring TNF bound to endogenous anti-TNF antibodies then infliximab, which binds to individual TNF subunits, should deplete the assay signal ( Figure 7C )

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Factors determining TBI outcome get deregulated by NF-B activation in astrocytes Finally, we determined the protein expression status of top-regulated factors associated with disease progression and regeneration at day 3 post-TBI, including TGM1 ( Tgm1 ), HO-1 ( Hmox1 ), and OPN ( Spp1 ), which are known to be involved in protective and remodeling processes after brain injury 48,49,50