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After 30 min incubation with 100 g/ml of RNase A and 10 g/ml of PI staining solution in the dark, the cells were analyzed using a FACScan flow cytometer (Becton Dickinson, USA)

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For instance, due to the poor bioavailability of NAC, the systemic levels following oral NAC doses are in the nanogram to microgram range, whereas, due to the high conversion of orally administered NAC to cysteine in addition to endogenous levels, the concentrations of cysteine are in the microgram to milligram range

As a therapy of already established adjuvant arthritis, the salutary effect of NaBPC157 consistently appeared after only two weeks of medication and it was clearly evident after one year of application