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Gereben, B
Capsaicin-induced activation of p53-SMAR1 auto-regulatory loop down-regulates VEGF in non-small cell lung cancer to restrain angiogenesis
This action inhibits ferroptosis in H9C2 cells in vitro and alleviates SIC in vivo in a dose-dependent manner, suggesting a potential treatment strategy for SIC (Wang et al., 2023a

Tissues were permeabilized with PBS supplemented with 0.1% Tween-20 for 10 min, followed by a peroxidase block with 3% H 2 O 2 (Sigma-Aldrich, 216763) for 20 min

The study also found that hydrogen-rich water alleviates intestinal oxidative stress by upregulating the expression of SIRT1, Nrf2, and HO-1, thus activating the SIRT1/Nrf2/HO-1 signaling pathway, which further improves constipation