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2.5 RNA-seq RNA-seq was performed by BGI genomics ( Total RNA was extracted from approximately 100 mg of rosette tissue using the SIGMA Spectrum Plant total RNA KIT (1003037777) according to the manufacturers instructions with on-column DNase treatment

The pathogen-associated -glucans are insoluble chains of D-glucose linked by 13 glycosidic bonds, with some 16 branching in yeast and fungi

Vitamin B12 is found in animal products such as grass-fed beef, wild-caught fish, eggs from pastured chickens, and grass-fed, raw cheeses

Vines were the subject to various levels of N status, while vine water status and vigor were controlled

These results indicate that CSE treatment did not significantly impact barrier integrity, but led to increased cytotoxicity, elevated inflammatory markers, and heightened oxidative stress