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Description
Vector and insert fragments were linearized by polymerase chain reaction (PCR) and template DNA was digested with DpnI
It requires larger injection volumes, which some users actually prefer because drawing a bigger volume is easier to measure accurately on a standard syringe
Mention prior reactions to vitamin B12, cobalt, or injections
doi: 10.1038/nsmb.3194
These studies are, of course, not all straightforward, and measurement of the broccoli-derived ingredients can be a source of mystery, obfuscation, and confusion, that is readily exploited by the supplement industry
