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After washing, 50 L/well of HRP-conjugated detection antibody anti-Ig-HRP (Cytiva NA9310-1ML) was added for 1 h at RT

Following the renaissance and promotion of TCM, mounting studies currently spotlight the seminal applications of herbs in the prevention and treatment of skeletal lesions and envisage their potential mechanisms via the gutbone axis

Nutrients 10, 1494

Nature 581, 221224

The latter then donates an amino group to an alpha-keto acid, alpha-ketoglutarate, to form an amino acidpyridoxal 5-phosphate Schiffs base intermediate that is then reduced to form glutamate
