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Description
Blood was collected by cardiac puncture and allowed to clot for 30 min at room temperature

J., Berry M

The separation via HPLC follows an isocratic method at 30 C using a reversed-phase column in two runs

You must expect some level of swelling, numbing and bruising since it is a surgery after all

[DOI] [PubMed] [Google Scholar] 85.Zeisel S.H., Mar M.H., Zhou Z., da Costa K.A