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Description

This reagent reacts with the thiol groups of GSH which convert 2-nitro-5-thiobenzoate (NTB ) to NTB 2 , thereby producing a yellow color, which can subsequently be quantified ( Glutathione Peroxidase GPx is measured by adding glutathione reductase (GR) to the sample after which nicotinamide adenine dinucleotide phosphate (NADPH) is transformed to a reduced state, allowing a decrease in absorbance to be subsequently measured (32)

In cells, iron is typically bound in complexes such as heme, ironsulfur clusters (enzyme cofactors), and ferritin, with only a small portion existing as the labile iron pool (LIP) [23]

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