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The MS conditions for the hydrophilic mushroom samples were as follows: LC-IM-QTOF-MS was run in positive ionization mode, scan rate 2.0 spectra/second, mass range m/z 501700, gas temperature 300 C, gas flow 12.0 L/min, nebulizer 35 psi, skimmer 65 V, capillary voltage 3500 V, fragmentor 100 V, reference masses 121.050873 and 922.009798 (Reference mix, Agilent Technologies)

For SDS-PAGE, 5-20 g of total protein was used and semi-dry blotted on PVDF membranes

Z.VuleticL

Thus, the unmodified M pro appeared to behave as a typical monomer/dimer two-species system with dimerization dependent on concentration, while glutathionylated M pro behaved essentially as a single monomer-like species independent of its concentration