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J Immunol 155:21232133 Fowlkes JL, Serra DM, Bunn RC et al (2004) Regulation of insulin-like growth factor (IGF)-I action by matrix metalloproteinase-3 involves selective disruption of IGF-I/IGF-binding protein-3 complexes

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Blood samples (100 L each) were collected from mice via cardiac puncture and allowed to clot at room temperature for 30 min