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This tightly regulated molecular process ensures that gas exchange is continuous and efficient, adapting to the varying demands of the body

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Eluted protein was further purified by HiTrap Heparin HP affinity column (Cytiva, 17040703) with a linear gradient of Low-Salt Wash Buffer (50 mM HEPES-NaOH pH 7.4, 50 mM NaCl, 2 mM EDTA, 10% Glycerol, and 2 mM DTT) and High-Salt Wash Buffer (50 mM HEPES-NaOH pH 7.4, 1 M NaCl, 2 mM EDTA, 10% Glycerol, and 2 mM DTT) to remove RNA contamination

Funding This work was supported by grants from the National Institute of Health (NIH) (K.H.) R01NS094756 and National Institute of Neurological Disorders and Stroke (NINDS)

April 2024