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Even when a combination of droplet printing, conduction cooling and laser warming is used to dramatically increase the droplet vitrification volume(best for 4 uL)(Fig

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Crystallization, data collection, and structural determination Crystallization of the complexes of MavL-ADPR, MavL 40-404(D315A) -ADPR-Ub, and LnaB-Actin were conducted using the hanging-drop vapor diffusion method at 16 C, with drops containing 0.5 l of the protein solution mixed with 0.5 l of reservoir solution