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Because the latter assay is conducted in a cuvette, as opposed to our microtiter plate assay, we also included a third assay, which is based on the 2VP masking in a microtiter plate [11]

5b) and at least delay reproductive potential (Supplementary Fig

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The PYR assay consists of three steps (Harrington et al., 2013): Biotinylated primers are used for PCR amplification and labeling. Streptavidin separates the PCR products and hybridizes to sequencing primers. Sequencing is performed, with nucleotides added one by one according to the template sequence