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Rotate between these sites weekly to minimize injection site reactions and prevent lipodystrophy (changes in the fat tissue at the injection site)

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Template cDNAs were obtained by reverse transcription of total RNAs using an oligo (dT) primer and a PrimeScript RT reagent Kit (TAKARA, Japan)

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Endothelin 1 transcription is controlled by nuclear factor-kappaB in AGE-stimulated cultured endothelial cells