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doi: 10.1111/j.1749-6632.2001.tb02729.x 282

It is done as an out-patient procedure and you need not take a day off from work

The dataset was then imported into R software [R version 3.4.2] and normalized using log transformation for multivariate analysis, unsupervised principle component analysis (PCA), and partial least squares-discriminant analysis (PLS-DA)

Accepted: February 11, 2025

This was warranted because only three samples per treatment were used in the initial RNA-Seq experiment, which may have affected overall false-positive rates and statistical power
