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A priori power analysis (G*Power 3.1) determined that a minimum of 5 mice per group was required to achieve 80% statistical power with an -level of 0.05, assuming a medium effect size (f = 0.25) based on preliminary data

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3.4 Tryptophan metabolites Tryptophan is an essential amino acid that is not only a building block for protein synthesis but also serves as a precursor for neurotransmitters and other bioactive substances, such as serotonin and melatonin, which have a significant impact on the gut-brain axis (66)