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Thermal cycling conditions were 15 cycles of 37 C for 5 min and 20 C for 5 min, followed by 55 C for 30 min for complete backbone digestion

[DOI] [PubMed] [Google Scholar] 15.da Silva Schmidt P.M., Trettim J.P., Longoni A., Grings M., de Matos M.B., de Avila Quevedo L., Ardais A.P., Nedel F., Ghisleni G., Leipnitz G., et al

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Although rheumatic fever has been attributed to molecular mimicry for half a century no antigen has been formally identified (if anything too many have been proposed)