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Thiol and thioester quantification Cells were treated as indicated for individual experiments, after which media were aspirated and cells were washed 2 with 1 mL of ice-cold Hanks balanced salt solution (HBSS)

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Therefore, we conducted a peptide depletion experiment with FITC and the lysine peptide (10:1) using the same conditions as for the other isothiocyanates (MeOH/phosphate buffer pH 7.5)

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