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We have overcome the requirement to pattern hPSCs in a 2D format to definitive endoderm 3 by directly patterning in 3D pluripotent aggregates in suspension in an extracellular matrix (ECM)- and growth factor-independent manner

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The effect of vitamin B12 deficiency is hyperhomocysteinemia, which predisposes to the development and deepening of atherosclerotic changes and increases the risk of circulatory system diseases, nervous system diseases, and cancers [4,24,35,48,49]
So, there are only a few studies aimed at determining the beneficial properties of the modified analogues of BPC 157 [31]

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