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Establishment of normal concentrations of NAD and Glutathione forms in Whole Blood Next, we applied our profiling methodology to define human population values of the six redox metabolites using frozen whole blood samples from 299 anonymous healthy donors (191 females and 108 males, aged 18-70 years) provided by the Finnish Blood Service BioBank and 38 healthy in-house volunteers

Therefore, the observation that nitrotyrosine adducts are almost exclusively found inside mitochondria 18 suggests that superoxide is generated within the mitochondria and is not derived from other cellular sources

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