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Table 5 Supercritical extraction parameters and results applied to phytochemicals in some NUS species
Apoptosis assays Unless otherwise stated, the cancer cells were stimulated with TNF- (10 ng/ml) plus cycloheximide (10 g/ml) for 6 h
Following the addition of a colored reagent and a 5 min incubation at 37 C, a second absorbance reading was taken
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On the other hand, using menadione as a substrate, a fungal GHR proved to deglutathionylate both forms with similar rates, but it is more efficient with the reduced forms because of a much better affinity (Meux et al., 2011)
