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doi: 10.1007/s10741-021-10106-9 134 SciacquaASuccurroEArmentaroGMiceliSPastoriDRengoGet al

The solvent system in C18-RP ESI+ was A = 0.1% formic acid in water, and B = 0.1% formic acid in acetonitrile

Finally, 20 l of n-butanol supernatant containing MDA-TBA was injected into the HPLC device (HPLC-Agilent 1200, USA), and then, the amount of each sample was calculated using a standard curve

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GSNO reductase (GSNOR) activity assay Cell lines were washed, trypsinized and homogenized by sonication (30 in ice) in a solution containing 20 mM Tris-HCl (pH 8.0), 0.5 mM EDTA, 0.1% NP-40 and 1 mM phenylmethylsulphonyl fluoride (PMSF)