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[DOI] [PMC free article] [PubMed] [Google Scholar] 133.Kim H.H., Kang Y.-R., Lee J.-Y., Chang H.-B., Lee K.W., Apostolidis E., Kwon Y.-I

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For one-step reactions, all components were mixed in 96-well microtitre plates in 100 l volume (PS, F-Bottom, clear, Greiner) and reactions were performed for 30 min in a Spark 20 M (Tecan) plate reader at 30 C

Each production lot is independently characterized using high-performance liquid chromatography (HPLC) and liquid chromatographymass spectrometry (LCMS) protocols